ABSTRACT
OBJECTIVE: To determine the association of TNF alpha and NRAMP1 polymorphisms in leprosy. MATERIAL AND METHOD: The polymorphisms of TNF alpha at -238, -308, and NRAMP1 at INT4, D543N, and3' UTR were examined in 37 patients with leprosy (24 multibacillary and 13 paucibacillary) and 140 healthy controls. PCR-SSP and PCR-SSO method were used to type TNF and NRAMP1 polymorphisms. RESULTS: The genotype frequency of TNF-308 G/A was significantly increased in all leprosy patients compared to the controls (p = 0.04, OR = 2.69). When leprosy types were divided, the allele frequency of TNF-308A was significantly increased in multibacillary leprosy compared to the normal controls (p = 0.04, OR = 2.93). There was no significant difference in the distribution of the genotypes and allele frequencies of TNF -238 and NRAMP1 polymorphisms between the patients and controls. CONCLUSION: TNF-308A was associated with susceptibility to multibacillary leprosy.
Subject(s)
Alleles , Case-Control Studies , Cation Transport Proteins/genetics , Disease Susceptibility , Genotype , Humans , Leprosy/genetics , Polymorphism, Genetic , Risk Factors , Thailand , Tumor Necrosis Factor-alpha/geneticsABSTRACT
OBJECTIVE: To investigate the association between HLA class II alleles and autoimmune hepatitis (AIH) type I in Thai patients. MATERIAL AND METHOD: The clinical data of 50 autoimmune hepatitis patients type I (AIH) at Siriraj hepatitis clinic were analysed, 37 of whom were tested for HLA class II genotyping using polymerase chain reaction and sequence-specific oligonucleotide technique (PCR-SSO). RESULTS: AIH is an uncommon chronic hepatitis in Thailand with females predominant. The HLA DRB1*0301, and DQA1*0101 were significantly associated with AIH patients when compared to controls; (OR = 3.92 [1.18-13.30], p 0.021, OR = 2.31 [1.13-4.73], p 0.019, respectively). When 18 patients with "definite" AIH were analysed, only HLA DRB1*0301 was still significantly associated with AIH (OR = 5.22, 95%CI = 1.28-20.92, p 0.015). CONCLUSION: HLA genotyping has shown that HLA DRB1*0301 and HLA DQA1*0101 were significantly associated with AIH.
Subject(s)
Adult , Aged , Aged, 80 and over , Autoimmune Diseases/epidemiology , Epidemiologic Studies , Female , Genotype , HLA Antigens/genetics , HLA-DQ Antigens/genetics , HLA-DR Antigens/genetics , Hepatitis, Autoimmune/epidemiology , Histocompatibility Antigens Class II/genetics , Humans , Male , Middle Aged , Polymerase Chain Reaction , Polymorphism, Genetic , Risk Factors , Thailand/epidemiologyABSTRACT
Serum samples from 49 patients with panel reactive antibodies of greater than 15% and 17 patients who have related donor pairs were collected at the Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital. Crossmatching was performed by three methods, flow cytometry crossmatch (FCXM), the standard National Institutes of Health (NIH), and the antihuman globulin (AHG) microlymphocytotoxicity. 28.9% Spell out of both T- and B-cell crossmatch was positive by FCXM and negative by NIH and AHG. When the T-cell and B-cell crossmatches were negative by FCXM, they were negative by both NIH- and AHG method. There was significant difference of the crossmatch result between FCXM and NIH and between FCXM and AHG (p < 0.0001). In addition, FCXM was about 4-16 and 8-32 times more sensitive than AHG- and NIH method, respectively. In conclusion, the result of FCXM is clear and this method is more sensitive than NIH- and AHG method FCXM should be used together with the NIH- and AHG method for kidney transplantation.